Identification of a Rice Leaf Width Gene Narrow Leaf 22 (NAL22) through Genome-Wide Association Study and Gene Editing Technology
文献类型： 外文期刊
作者： Xu, Yuchen 1 ; Yan, Shuangyong 3 ; Jiang, Su 1 ; Bai, Lu 2 ; Liu, Yanchen 2 ; Peng, Shasha 1 ; Chen, Rubin 2 ; Liu, Qi 2 ; Xiao, Yinghui 1 ; Kang, Houxiang 2 ;
作者机构： 1.Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
3.Tianjin Acad Agr Sci, Tianjin Crop Res Inst, Tianjin Key Lab Crop Genet Breeding, Tianjin 300112, Peoples R China
关键词： Cell division; CRISPR; Cas9 gene editing; Genetic architecture of rice leaf width; Genome-wide association study; Gibberellin; Rice leaf width; Vein width
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 24 卷 4 期
页码：
收录情况： SCI
摘要： Rice leaf width (RLW) is a crucial determinant of photosynthetic area. Despite the discovery of several genes controlling RLW, the underlying genetic architecture remains unclear. In order to better understand RLW, this study conducted a genome-wide association study (GWAS) on 351 accessions from the rice diversity population II (RDP-II). The results revealed 12 loci associated with leaf width (LALW). In LALW4, we identified one gene, Narrow Leaf 22 (NAL22), whose polymorphisms and expression levels were associated with RLW variation. Knocking out this gene in Zhonghua11, using CRISPR/Cas9 gene editing technology, resulted in a short and narrow leaf phenotype. However, seed width remained unchanged. Additionally, we discovered that the vein width and expression levels of genes associated with cell division were suppressed in nal22 mutants. Gibberellin (GA) was also found to negatively regulate NAL22 expression and impact RLW. In summary, we dissected the genetic architecture of RLW and identified a gene, NAL22, which provides new loci for further RLW studies and a target gene for leaf shape design in modern rice breeding.
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